Generation of a ceramide synthase 6 mouse lacking the DDRSDIE C-terminal motif
Fig 1
Generation of CerS6ADAAAIA mice.
The CerS6 genomic region showing the CRISPR/Cas9 design for editing the CerS6 DDRSDIE motif. Genomic location (mm10 chr2:68,861,441–69,114,282) of the guide RNA cleavage site is shown as a dashed line. The single strand (ssDNA) repair oligo spanning 60 base pairs of the genomic sequence up- and down-stream of the edited motif is indicated. The genomic and repair sequences are shown, and amino acid residues that were modified are in red. Insert, mouse kidney homogenates (20 μg of protein) were analyzed by Western blotting using an anti-CerS6 antibody, repeated twice with similar results. Mr markers are shown. GAPDH was used as a loading control.