Midazolam impacts acetyl—And butyrylcholinesterase genes: An epigenetic explanation for postoperative delirium?
Fig 4
KDM1A mRNA expression was quantified relative to β-Actin mRNA expression by qPCR.
Increased expression of lysine specific demethylase (KDM1A) in different cells after midazolam [50 μg/ml] exposure for 24 hours analyzed by qPCR. KDM1A expression increased in U343 (n = 3; A, in peripheral blood mononuclear cells (PBMCs) (n = 8; B) and in SH-SY5Y (n = 3; C). Flumazenil did not reduce KDM1A expression (n = 6, D). Data are presented as mean ± standard deviation. The reported p-value refers to the Dunnett’s post-hoc test, comparing the underlying columns at the ends of each bar.