Midazolam impacts acetyl—And butyrylcholinesterase genes: An epigenetic explanation for postoperative delirium?
Fig 1
Activity and mRNA expression of AChE and BChE after incubation with midazolam A) the intracellular cholinesterase activity increased 24 h after midazolam exposure (n = 4; p = 0.01) and was measured by fluorometric assay B) ACHE mRNA quantified by qPCR expression was increased 24 hours after midazolam exposure in SH-SY5Y cells (n = 3; p<0.01) C) BCHE mRNA quantified by qPCR expression was increased 24 hours after midazolam exposure in SH-SY5Y cells (n = 3; p = 0.03). Data are presented as mean ± standard deviation. The reported p-value refers to the Dunnett’s post-hoc test, comparing the underlying columns at the ends of each bar.