Cytokine and phenotypic cell profiles in human cutaneous leishmaniasis caused by Leishmania donovani
Fig 3
Comparison of selected cytokines production by PBMC derived dendritic cells stimulated with SLA.
Dendritic cells from LCL (n = 20), healthy non endemic controls (n = 10) and healthy endemic controls (n = 20) were stimulated with L. donovani antigen (50μg/ml). The supernatant was harvested at 24, 48 and 72 hours and intracellular (A) IL-12p70 and (B) IL-10 levels were determined by flowcytometry(in duplicate). The box represents the IQR (i.e. the middle 50% of the observations) with the horizontal line representing the median. The whiskers represent the main body of the data, indicating the range of the data. Cytokine levels were compared using nonparametric Kruskal-Wallis test followed by Dunn’s multiple comparison test (*p<0.05; **p<0.01; ***p<0.001). (C) IL-12p70 and IL-10 levels showed a strong positive correlation at 72 h (Spearman correlation test, p< 0.05).