Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos
Fig 1
Single-nucleus RNA-sequencing analysis of pre-cellularization Drosophila melanogaster embryos (A) Two-dimensional UMAP embedding of nuclei shows 10 transcriptionally distinct clusters, as determined using the Leiden algorithm. Associated colors are maintained throughout the manuscript. (B) Two-dimensional UMAP embedding of nuclei labeled by condition shows the overlap of control and dCTCFmat-/- nuclei in a reduced dimensional space following correction for non-biological variability. Associated colors are maintained throughout the manuscript. (C) Heatmap of scaled gene expression for top four marker genes of each cluster, clusters are hierarchically ordered.