Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum
Table 4
High efficiency of gene deletion via RNP transformation was tested by FGSG_04274 for verification.
Deletion mutants were identified by the dark red color and abnormal shapes of the colonies. *50 bp indicates that GEN is amplified by 70 bp primers, which contain 50 bp arms homologous to sequences upstream or downstream from FGSG_04274.