Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum
Fig 3
Construction of deletion mutants by Cas9 RNP-mediated transformation with different homology arm sizes.
(a) Schematic representation of PKS12 deletion. Three constructs with different homology arm sizes (1 kb, 270 bp, and 50 bp) were generated for PKS12 deletion. (b) Colony morphologies of F. graminearum strains generated during Cas9 RNA-mediated transformation. (c) Each randomly chosen plate of disrupted phenotypes was counted as transformants in regeneration media. Expected phenotypes were later calculated as frequencies.