Effects of arsenic on the topology and solubility of promyelocytic leukemia (PML)-nuclear bodies
Fig 8
Effects of TAK243 (ubiquitination inhibitor) and ML792 (SUMOylation inhibitor) on the number and size of PML–NBs in CHOGFPPML (A) and HEKGFPPML cells (B). The cells were cultured in the presence of 0.1% DMSO (control), 10 μM TAK243 for 4 h, or 20 μM ML792 for 4 and 24 h. The number of PML–NBs in both cell types decreased and their sizes increased significantly by treatment with ML792. TAK243 was less effective than ML792 in decreasing the number of PML–NBs, and the cells looked shrunken and slightly damaged after 6 h of culture with 10 μM TAK243. Data are presented as the mean ± SEM. *, Significantly different from control group. #, Significantly different from all other groups. &, Significantly different from control and TAK243 groups. Scale bar = 10 μm.