Conditioned medium from human tonsil-derived mesenchymal stem cells inhibits glucocorticoid-induced adipocyte differentiation
Fig 6
T-MSC CM promotes platelet production.
(A) Red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs) in mouse peripheral blood were counted using an auto hematology analyzer on the day of sacrifice. Data are presented as mean ± SEM and were analyzed using one-way ANOVA (n = 5, **P < 0.01). (B) Immunohistochemical analysis of megakaryocytes in mouse bone marrow. Representative images with a higher magnification in the lower panel (200× original magnification). (C) K562 human lymphoblasts were induced for megakaryocytic differentiation in the presence or absence of T-MSC CM. Representative images of cells with Giemsa staining (200× original magnification). (D) Megakaryocyte differentiation was induced with or without T-MSC CM, and the effects of OP9 adipocyte co-culture were examined. Expression levels of megakaryocyte marker CD41 were determined using flow cytometry. Data are presented as mean ± SEM and were analyzed using one-way ANOVA (n = 3; *P < 0.05, ***P < 0.001).