Vocal changes in a zebra finch model of Parkinson’s disease characterized by alpha-synuclein overexpression in the song-dedicated anterior forebrain pathway
Fig 8
Asyn overexpression differentially affects across-rendition variability in select acoustic features depending on syllable type.
The adjusted value of across rendition variation (i.e., CV, rendition-to-rendition variability) in select acoustic features is plotted for all individual syllables sung by ASYN (purple) and GFP (green) groups. Overall, the CV of amplitude (mean.amplitude.adjusted) for syllables sung by the ASYN group was lower than the GFP control group at 2 mpi (top left). Additionally, the CV of pitch goodness (mean.pitch.goodness) was higher in the ASYN group than in the GFP group at 3 mpi (top middle). The adjusted value of across rendition variation in select acoustic features is also plotted for Harmonic (middle left), Noisy (middle center), Mixed (middle right), and Slide (bottom) syllables. For harmonic syllables, the CV of entropy (mean.entropy) was significantly higher in the ASYN group compared to GFP control at 1, 2, and 3 mpi. For noisy syllables, the CV of amplitude (mean.amplitude.adjusted) was lower in the ASYN group than in the GFP control at 2 mpi. For mixed syllables, the CV of pitch goodness (mean.pitch.goodness) was higher in the ASYN group compared to the GFP control at 3 mpi For slide syllables, the CV of amplitude (mean.amplitude.adjusted) was lower in the ASYN group compared to GFP control at 2 and 3 mpi (bottom left). The CV of pitch (mean.pitch) was higher for the slide syllables in the ASYN group compared to GFP control at 3 mpi with a trend observed at 1 mpi (bottom middle). The CV of frequency modulation (mean.FM) for slide syllables was lower in the ASYN group compared to GFP control at 1 mpi (bottom right). Remaining acoustic features whose CV scores were not affected by αsyn overexpression are reported in S9-11. Summary statistics provided in Table 6. Reference Fig 7‘s legend for explanation of boxplots. Statistical comparisons were made using a Wilcoxon Rank Sum Test. * indicates p < 0.05. # indicates 0.05 < p < 0.1.