RespiCoV: Simultaneous identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and 46 respiratory tract viruses and bacteria by amplicon-based Oxford-Nanopore MinION sequencing
Fig 1
Correlation of SARS-CoV-2-specific reads obtained by RespiCoV (shown for three different sequencing runs) and the Cq value generated by a specific real-time PCR assay.
Even if accurate quantification by RespiCoV is questionable, within one run the correlation is significant (R squared: 0.88, 0.96, 0.91, and 0.99 for run 1, run 2, run 3, and the INSTAND samples, respectively).