Characterization of intracellular calcium mobilization induced by remimazolam, a newly approved intravenous anesthetic
Fig 3
Characterization of remimazolam-induced intracellular calcium elevation in the absence of extracellular calcium.
After eliminating calcium from the extracellular buffer, remimazolam-induced elevation of intracellular calcium was observed at concentrations between 0 and 500 μM in SHSY-5Y cells and HUVECs. (A) Remimazolam (300 μM)-induced elevation of intracellular calcium was not influenced by the elimination of extracellular calcium in SHSY-5Y cells (n = 3–7, * p < 0.05, compared to control, one-way ANOVA followed by Dunnett’s post-test). The same concentration of DMSO corresponding to 500 μM of remimazolam was administered as a control. The horizontal line in each box indicates the median, the box shows the interquartile range (IQR), and the whiskers represent 1.5 × IQR. (B) Remimazolam (300 μM)-induced elevation of intracellular calcium was not influenced by the elimination of extracellular calcium in HUVECs (n = 3–7, * p < 0.05, compared to control, one-way ANOVA followed by Dunnett’s post-test). The same concentration of DMSO corresponding to 500 μM of remimazolam was administered as a control. The horizontal line in each box indicates the median, the box shows the interquartile range (IQR), and the whiskers represent 1.5 × IQR.