Characterization of intracellular calcium mobilization induced by remimazolam, a newly approved intravenous anesthetic
Fig 1
Remimazolam-induced intracellular calcium elevation.
(A) An example of a typical remimazolam-induced intracellular calcium elevation time series in HUVECs. Approximately one min after the administration of 300 μM remimazolam, an increase in the fluorescence intensity of Fluo-4 was observed, indicating that intracellular calcium was increased. Fluorescence intensity in the circled area was used as background. (B) The method of semi-quantifying the rate of calcium elevation. The red line indicates the average fluorescence change over the entire observed region. The blue line indicates the fluorescence change in the background region without cells. The differences between both lines before the remimazolam application (b) and at the peak of calcium elevation (a) were measured. The ratio a to b (a/b) was considered as an index of remimazolam-induced calcium elevation.