Isolation of extra-cellular vesicles in the context of pancreatic adenocarcinomas: Addition of one stringent filtration step improves recovery of specific microRNAs
Fig 4
Expression of miR-1246 in OP1 and OP2 groups of EVs derived from the plasma of xenografted nude mice and PDAC patients/healthy controls.
Plasma were collected from xenografted mice at 1 and 2 weeks post PANC-1 inoculation, and from patients with early stage PDAC and healthy controls. Top. qRT-PCR (80ng total RNA used for each cDNA synthesis) analysis of miR-1246 expression in the OP2 (left) and OP1 (right) group of EVs derived from the plasma of xenografted nude mice and control mice (n = 6). Middle. Tumor volume (left) and mice weight (right) are shown. Low. qRT-PCR (80ng total RNA used for each cDNA synthesis) analysis of miR-1246 expression (top panel) and miR-196a expression (low panel) in the OP1 and OP2 group of plasma EVs between PDAC patients and healthy donors (control). *, p<0.05, unpaired Student t-test (n = 7). Individual values of detection are presented in the supplement material.