PKI-587 enhances radiosensitization of hepatocellular carcinoma by inhibiting the PI3K/AKT/mTOR pathways and DNA damage repair
Fig 1
PKI-587 blocked PI3K/AKT/mTOR induced by radiation and inhibited the DNA damage repair pathway when combined with radiation.
(A and B) Western blot assay and semiquantitative analysis of the levels of PI3Kp110α, PI3Kp110γ, p-Akt (Ser473), Akt, p-mTOR (Ser2448), and mTOR proteins involved in PI3K/AKT/mTOR pathway in SK-Hep1 cells after radiation and PKI-587 alone or in combination treatment for 24 h. (C and D) Western blot assay and semiquantitative analysis revealed the levels of related proteins in DNA damage repair pathway, including p-DNAPKcs (Ser2056), DNAPKcs, p-ATR (Ser428), ATR, p-ATM (Ser1981), and ATM in SK-Hep1 cells after radiation and PKI-587 alone or in combination treatment for 24 h. The data are mean ± SD, n = 3. *P<0.05, **P<0.01, ***P<0.001; n.s: no significance. versus IR group. IR: ionizing radiation (6MV-X ray).