Molecular interaction of nitrate transporter proteins with recombinant glycinebetaine results in efficient nitrate uptake in the cyanobacterium Anabaena PCC 7120
Fig 2
Nitrate uptake in Anabaena 7120, WT and ApGSMT-DMT transformant.
Exponential phase cyanobacterial cells growing on N2 in the presence and absence of 0.1 M NaCl were collected and suspended in their specific media containing varied concentrations (40–500 μ) of nitrate. Uptake was performed under standard growth conditions in light (70 μE m2 s1). Samples were withdrawn at 10 min of incubation and measured nitrate depletion in the incubation mixture. Values represent means and SDs of triplicate experiments. (A) Initial rate of nitrate transport at different substrate concentrations; (B) Hofstee plot (v versus v/[S]) to determine the kinetics.