Interactome analysis of Bag-1 isoforms reveals novel interaction partners in endoplasmic reticulum-associated degradation
Fig 2
Components of isoform-specific Bag-1 complexes identified by BN PAGE/LC-MS/MS.
a. Experimental workflow. Bag-1 immunoprecipitates were run on native gel. Individual bands in each lane were cut, trypsinized and the resulting peptides were analyzed by LC-MS/MS. M: Protein marker. b-d. Seven protein complexes were characterized for Bag-1S (b), five for Bag-1M (c), and six for Bag-1L (d). Identified proteins are color-coded as following: Blue: Bag-1, green: Chaperones, yellow: ER-resident proteins, orange: Ubiquitination elements, purple: Shuttling proteins, red: Proteasome subunits.