Interactome analysis of Bag-1 isoforms reveals novel interaction partners in endoplasmic reticulum-associated degradation
Fig 1
Bag-1 isoform-specific interactome analysis and classification of enriched pathways.
a. Schematic representation of TAP–Bag-1 constructs used in the interactome analysis. TAP: Tandem Affinity Purification, NLS: Nuclear localization signal, TRSEEX: Amino acid repeats, UbL: Ubiquitin-like domain, BAG: BAG domain. b. Venn diagram of candidate interaction partners enriched (>1.2 fold) in the Bag-1 isoforms’ interactomes relative to the mock interactome. c. Subset of Gene Ontology (GO) classifiers for candidate Bag-1-interactors. Percentage of proteins with the indicated GO terms within Bag-1 interactomes and the reference dataset are depicted. Blue: Bag-1S, green: Bag-1M, purple: Bag-1L and grey: Reference. d. Heatmap analysis for the relative abundances of the binding partners for each Bag-1 isoform. Enriched proteins are clustered according to their biological functions. Color code is given in log2 base.