Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves’ orbitopathy
Fig 4
Effect of transforming growth factor (TGF)-β1, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α on fibroblast growth factor 10 (FGF10) expression in Graves′ orbitopathy (GO) and non-GO orbital fibroblasts (OFs).
(A) Representative western blot images showing that TGF-β1, IL-1β, and TNF-α increased expression of FGF10 at various time points (0–72 h) in both GO and non-GO OFs. Bar graphs show the relative density of each protein normalized to the level of β-actin and are represented as means ± standard deviation. (B) Expression levels in GO OFs of secreted FGF10 protein in response to TGF-β1, IL-1β, and TNF-α, using ELISA. Experiments were performed at least three times using different strains. *p < 0.05.