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Spectral dependency of the human pupillary light reflex. Influences of pre-adaptation and chronotype

Fig 4

Experimental data and circumstances.

(A) Normalized pupillary constriction (nPC) plotted against wavelength, for each of the nine protocols. The color scale shows at which point in the series a specific wavelength was presented; light yellow represents early in the series. For all but the Short protocols, points represent the average nPC during the respective last five seconds of a light step. In the two Short protocols, points represent the average nPC during the sixth second after lights-off (or sevenths second after lights-on). Traces show the mean nPC, ribbons its standard deviation. The number in the upper right corner of each plot shows the corresponding sample size. (B) Scatterplot of when subjects of a certain chronotype started their respective protocols. The color scheme is according to (C), i.e. green, red, and blue correspond to Exp. I–III, respectively. Note the lack of Lark and Owl chronotypes in Experiment I before midday. (C) Boxplot of the time of the year when the experiments took place. Preliminary measurements and parts of Experiment I took place in 2019, the other measurements in 2020.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0253030.g004