Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes
Fig 3
Oil Red O staining showing 3T3-L1 preadipocyte cells differentiated into mature adipocytes and effects B. spergulifolius extracts on lipid accumulation in 3T3-L1 cells.
A. The microscopic images at 10X magnification of lipid droplet of preadipocytes, mature adipocytes without B. spergulifolius extracts and adipocytes treated with IC50 dose of MeOH, EA and Aqueous of B. spergulifolius extracts stained with Oil Red O (Scale bar: 100 μm) B. The relative lipid quantization of preadipocytes, mature adipocytes treated without B. spergulifolius extracts and with MeOH, EA, Aqueous of B. spergulifolius extracts. OD490 of Oil Red O staining from three independent experiments was measured. Data are expressed as the relative change of the OD490 and presented as the means ± SD. To analyze statistical was used One-way ANOVA test. *p< 0.0001 and **p< 0.001, significantly different from mature 3T3-L1 Adipocytes. ***p< 0.001, significantly different from Aqueous.