Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes
Fig 2
Live-dead staining showing the effect of B. spergulifolius extracts on cell viability in 3T3-L1 adipocytes.
Live-dead staining assay was performed preadipocytes, mature adipocytes without and mature adipocytes with treated with IC50 dose of MeOH, EA and Aqueous of B. spergulifolius extracts for 24 h A. Representative microscopic images at 10X magnification showed 3T3-L1 cells without and treated with B. spergulifolius extracts stained with Calcein-Acetomethoxy (C-AM) and Ethidium homodimer-1 (Eth-1). C-AM (green fluorescence) and Eth-1 (red fluorescence) staining showed respectively live and dead 3T3-L1 cells (Scale bar: 100 μm) B. Quantitative data of live-dead staining assay. Three coverslips for each cell group were evaluated. Six distinct-randomly chosen regions in each coverslip were visualized and analyzed using microscope and ImageJ software. Data are expressed as a total percentage of viable and dead cells and presented as the means ± SD. To analyze statistical was used Student’s t-test (two-tailed). *p< 0.05, significantly different from mature 3T3-L1 Adipocytes. **p< 0.05, significantly different from Aqueous.