Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes
Fig 1
MTT assay showing the effect IC50 value of B. spergulifolius extracts on 3T3-L1 cell viability.
A. Effect of B. spergulifolius extracts on 3T3-L1 cell viability. The mature 3T3-L1 adipocytes were treated for 24 h with increased concentration of Methanol (MeOH), Ethylacetate (EA), and Aqueous extracts of B. spergulifolius. Also, cell treated without crude plant extract was taken as a control. B-D. The half-maximal (50%) inhibitory concentration (IC50) value of the B. spergulifolius extracts. The IC50 value is calculated by non-linear regression model. Data expressed as the means ± SD from three independent experiments. To analyse statistically was used one-way ANOVA test. *p = 0.033, #p = 0.001 and **p< 0.001, significantly different from control.