Effect of NAD+ boosting on kidney ischemia-reperfusion injury
Fig 6
Effect of NAD+ boosting by NR on Klotho and Napi2c in the kidney.
Western blot of klotho protein in the kidney of sham+vehicle, sham+NR and IRI+vehicle and IRI+NR at 24 hours (A) and its quantification (B) normalized to actin. NAD+ boosting did not affect klotho levels in the sham groups. Klotho became significantly decreased by IRI in both the vehicle and NR groups after 24 hours. (C) Western blot of klotho in the kidney of IRI+vehicle and IRI+NR after 14 days and its quantification (D) showed that klotho in the IRI+NR 14 days group was significantly reduced as compared with the sham group. (E) The mRNA levels of Kl were similarly reduced at the protein level. (F) mRNA of Napi2c was significantly decreased 24 hours after IRI in both groups and administration with NR resulted in a further decrease. Napi2c: sodium phosphate cotransporter 2. Mean ± SD; * p < 0.05, ** p < .001, *** p < 0.0001.