Effect of NAD+ boosting on kidney ischemia-reperfusion injury
Fig 3
Effect of NR administration on NAD+ boosting.
NAD+, NADH levels and Sirtuin1 expression in the kidney. NAD+ and NADH levels were measured in the kidney of sham+vehicle, sham+NR and IRI+vehicle and IRI+NR at 24 hours after IRI or sham. (A-B) NAD+ and NADH levels were significantly elevated in NR groups compared with the sham groups. IRI+vehicle rats had a pronounced significant reduction in the NAD+ and NADH levels compared with the sham+vehicle group. Also, NADH levels were significantly decreased in the IRI+vehicle as compared with IRI+NR group. NR rescued the fall in NAD+ and NADH levels after IRI. (C) The NAD/NADH ratio was unchanged in all the groups. (D) The expression of Sirt1 was significantly increased in sham+NR rats. IRI resulted in a significant fall in Sirt1 levels to a similar level. Mean ± SD; * p < 0.05, ** p < .001, *** p < 0.0001 (n = 7 in each group).