Sprouty4 negatively regulates ERK/MAPK signaling and the transition from in situ to invasive breast ductal carcinoma
Fig 9
Stable overexpression of Sprouty4 in MCF10.CA1d cells reduces invasion.
(A) MCF10.CA1d control and Sprouty4 overexpressing cells (MCF10.CA1d/S1 and MCF10.CA1d/S2) were serum-starved overnight and allowed to invade though 1.5 mg/mL of matrix for 24 hours via Boyden chamber assay. Representative images of cellular invasion (scale bar = 500 μm). (B) Invasion was quantified and plotted using GraphPad Prism. A Kruskal-Wallis test employing Dunn’s test of multiple comparisons showed significant differences in invasion when comparing Sprouty4 overexpression lines to control (α = 0.01, 99% CI; ** p < 0.01). Whiskers represent the minimum and maximum values and bars represent the median. Data were collected from three independent experiments.