Sprouty4 negatively regulates ERK/MAPK signaling and the transition from in situ to invasive breast ductal carcinoma
Fig 7
Sprouty4 overexpression induces phenotypic reversion of MCF10.CA1d cells.
MCF10.DCIS, MCF10.CA1d control and Sprouty4 overexpressing cell lines (MCF10.CA1d/S1 and MCF10.CA1d/S2) were grown in 3D rBM overlay cultures. By 48 hours, structural differences were observed between the MCF10.CA1d and overexpression lines. Sprouty4 overexpressing cells exhibited phenotypic reversion and resembled the isogenic DCIS line more than the parental invasive line. Differential interference contrast images were captured on a Zeiss Cell Observer spinning disk confocal microscope using a 5x objective and stitching nine contiguous fields (3x3) together to ensure reproducibility; size bar = 500 μm. Images are representative of three independent experiments.