Sprouty4 negatively regulates ERK/MAPK signaling and the transition from in situ to invasive breast ductal carcinoma
Fig 4
Sprouty4 regulates and responds to the ERK/MAPK pathway.
The ERK/MAPK pathway was targeted in MCF10.CA1d cells cultured in 2D using three separate MEK1/2 inhibitors: U0126 (10 μM), PD184352 (100 nM) and MEK162 (100 nM) for 48 hours. U0124 (10 μM) is a negative control for U0126. Membranes were immunoblotted for Sprouty4, E-cadherin, Slug, phosphorylated ERK1/2 (pERK1/2), phosphorylated MEK1/2 (pMEK1/2), total ERK1/2, total MEK1/2, and β-tubulin. Expression levels are representative of three independent experiments.