A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis)
Fig 1
CPE and IFA of McCoy cells after Siamese crocodile Chlamydia infection.
(A) McCoy cells were infected with Chlamydia at 30°C. (B) McCoy cells were infected with Chlamydia at 37°C. CPE of uninfected (control cells) and infected McCoy cells was observed by phase contrast microscopy at 32× magnification (scale bar = 10 μm). For the IFA test, infected McCoy cells were detected with Chlamydiaceae family-specific mouse monoclonal antibody and observed by fluorescence microscopy at 40× magnification (scale bar = 50 μm). CPE and IFA at each temperature were observed at 24, 48, 72, 96, and 120 hpi.