ACRBP (Sp32) is involved in priming sperm for the acrosome reaction and the binding of sperm to the zona pellucida in a porcine model
Fig 1
Indirect immunolocalization of SERCA 2 in fixed and permeabilized porcine sperm.
Panel A indicates that acrosomal fluorescent labelling is evident when detected with SERCA 2 polyclonal antibody. Panel B shows the phase contrast microscopy photograph of the same field. Panel C shows the non-specific fluorescence of sperm labeled only with secondary fluorescein-conjugated rabbit anti-goat antibody. The experiment was repeated three times and representative photos are shown.