Identification of recombinant Fabs for structural and functional characterization of HIV-host factor complexes
Fig 8
Characterization of Fabs that bind the AP2 complex.
A. SEC profiles of AP2 core:CE1 Fab (black in upper panel), AP2 core:CE9 Fab (black in lower panel), AP-2 core alone (dashed orange) and Fab alone (dashed cyan) on a Superdex 200 10/300GL column. Single particle negative stain EM study of AP-2 core: CE1 Fab. B. or AP-2 core:CE9 Fab C. The left panel is 2D class averages for AP-2:Fab complex. The right panel showed that the atomic structures of a Fab (PDB: 3SQO and chain H,L in cyan) and an AP-2 core (PDB: 2VGL) were docked in the density map as rigid bodies (AP2 α in blue, β2 in orange, μ2 in magenta and σ2 in gray). D. Cartoon representation of Fabs (CE1 in upper panel, CE2 in lower panel) binding to AP-2 core. E. Representative negative stain 2D class averages of AP2 muCTD with BG12, CG7 or alone.