Age-related changes in NG2-expressing telocytes of rat stomach
Fig 1
Localization and characterization of NG2 cells in the stomach of adult rats.
(A-B) Localization of NG2 cells in the gastric mucosa. (A) Immunofluorescent images showing staining with both monoclonal NG2 (green) and polyclonal NG2 (red) antibodies and Hoechst (blue). (B) Magnified view of immunofluorescent images showing staining for NG2 (red) and Hoechst (blue). (C-D) Colocalization of CD34 (green) in NG2+ telocytes (red) of the adult stomach. Immunofluorescence staining for NG2 (red), Hoechst (blue) and CD34 (green) (a telocyte marker). (E-F) Co-expression of PDGFRα (green) in NG2+ telocytes (red) in the gastric mucosa. Immunofluorescence staining for NG2 (red) and PDGFRα (green) (another telocyte marker). Arrowheads show NG2+/PDGFRα+ telocytes whereas arrows point to NG2+/PDGFRα- telocytes. (G-H) Triple immunofluorescence staining for PDGFRα (green), CD34 (red) and NG2 (blue). Arrowheads indicate NG2+/CD34+/PDGFRα+ telocytes and arrows show NG2+/CD34+/PDGFRα- telocytes. Scale bars: 100 μm (A, C, E, G) and 20 μm (B, D, F, H).