Disruption of protease A and B orthologous genes in the basidiomycetous yeast Pseudozyma antarctica GB-4(0) yields a stable extracellular biodegradable plastic-degrading enzyme
Fig 3
Complementation of gene deletion in ΔPaPRO1 and ΔPaPRO2 mutants.
Secreted PaE in mutant strains (ΔPaPRO1 or ΔPaPRO2) bearing the plasmids pEmp-PaPRO1, pEmp-PaPRO2 or pEmp, and cultured for 8 days, were detected by immunoblotting with anti-PaE antibody. PC, purified PaE as a positive control; black triangle, full-length PaE; gray triangles, degraded fragment of PaE in strain GB-4(0).