Disruption of protease A and B orthologous genes in the basidiomycetous yeast Pseudozyma antarctica GB-4(0) yields a stable extracellular biodegradable plastic-degrading enzyme
Fig 2
PaE production in ΔPaPRO1 and ΔPaPRO2 mutants.
Wild-type and ΔPaPRO1 and ΔPaPRO2 gene-deletion mutants of strain GB-4(0) were cultured for 5 and 13 days. PaE and its degraded fragments in the supernatant were detected with anti-PaE antibody. PC, purified PaE as the positive control; black triangle, full-length PaE; gray triangles, degraded fragments of PaE in the strain GB-4(0).