Suppressive effects of vitamin C-treated induced-regulatory T cells on heart allograft rejection under vitamin C-deficient or –sufficient conditions
Fig 6
Impact of vitamin C on Foxp3 stability of V-iTregs and C-iTregs after heart transplantation in both WT and Gulo-KO mice.
(A) Proportion of Foxp3+ among transferred iTregs (GFP+CD4+) in the spleens of WT mice on day 7 following heart transplantation. (B) Proportion of total Foxp3+CD4+ Tregs in the spleens of WT mice on day 7 following heart transplantation. (C) Proportion of Foxp3+ among transferred iTregs (GFP+CD4+) in the spleens of Gulo-KO mice on day 7 following heart transplantation. (D) Proportion of total Foxp3+CD4+ Tregs in the spleens of Gulo-KO mice on day 7 following heart transplantation. Line and whiskers in the dot plots indicate the mean and SEM, respectively. *P < 0.05 compared to the control group by Student’s t-test. C-iTregs, the control induced-regulatory T cells without vitamin C treatment; Foxp3, Forkhead Box P3; GFP, green fluorescent protein; Gulo-KO, L-gulonolactone-γ-oxidase knockout; V-iTregs, vitamin C-treated, induced regulatory T cells; WT, wild type.