Leptin improves the in vitro development of preimplantation rabbit embryos under oxidative stress of cryopreservation
Fig 2
Data of in vitro expanding (A) and hatching (B) blastocyst rates of fresh and vitrified rabbit embryos cultured with different levels of leptin.
Bars represent the mean ± standard error (SE) and the different superscripts (a, b, c, d) indicate the significant differences between means (P<0.05). Number of initial cultured embryos per fresh/vitrified treatment group were 135/155, 109/100, 137/138 and 104/105 embryos for 0, 10, 20 and 100 ng/mL leptin groups, respectively. Expanding and hatching rates were calculated as percentages of expanded and hatched blastocysts from the initial cultured morulae embryos, respectively, in each treatment group.