Pitfalls in AR42J-model of cerulein-induced acute pancreatitis
Fig 1
Effect of dexamethasone and ceruleine on AR42J morphology.
Cells were incubated under standard conditions (DMEM supplemented with 10% fecal calf serum and 1% penicillin/streptomycin, upper image) with 48 hours of dexamethasone (dexa, middle) or 48 hours of dexa followed by 24 hours of cerulein (CN, lower image). Dexa led to a differentiation in an acinar like phenotype. Cell morphology was rounder and thicker with numerous granular vessels inside. Co-treatment of dexamethasone and CN resulted in similar morphologic alterations as did dexamethasone alone. Dexa: dexamethasone, CN: cerulein. Scale bars: 100μm.