Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
Fig 1
Expression and purification of His-tagged proteins.
(a) LEK, (b) GDH. M: Protein molecular weight maker, Lane 1: The whole cell lysates before induction, Lane 2: The whole cell lysates after IPTG induction, Lane 3: Soluble fraction of the whole cell lysates after IPTG induction, Lane 4–6: Eluates collected with 100, 200 and 500 mM imidazole, respectively.