Semaphorin-1a-like gene plays an important role in the embryonic development of silkworm, Bombyx mori
Fig 2
Structure diagram of the mutation region in Fuyin-lre.
In the Fuyin-lre mutant, the 281325 bp fragment starts from 11106950 bp and ends at 11388274 bp on chromosome 5 is replaced by some new DNA fragments. The 1232 bp and 1845 bp sequences were obtained at both ends of the mutation region by iPCR. 1–130 bp of the 1232 bp sequence and 1–186 bp of the 1845 bp sequence have multiple copies in the silkworm genome. 131–1232 bp of the 1232 bp sequence has only one single copy on the silkworm genome, which is derived from 11413374 bp to 11414475 bp of chromosome 5. A bmmar1 transposon was detected in 187–1845 bp of the 1845 bp sequence.