Novel functions of the luteinizing hormone/chorionic gonadotropin receptor in prostate cancer cells and patients
Fig 2
Effects of LH and hCG treatment on three prostate cancer cell lines (LNCaP, PC-3 and DU145).
(A) Dot plot showing the mRNA expression (ΔΔCt) of the LHCGR relative to RPS20 per cell line and type of stimulation. Expression of LHCGR is compared to the normal testis (dotted line). Solid line represents the mean expression of LHCGR. N = 3 of biological replicates, each dot consists of three technical replicates, 4μl cDNA loaded for each technical replicate. (B) Boxplot showing cell proliferation after stimulation with either LH or hCG at 1 IU/ml in the three prostate cancer cell lines. The box center represents the median, the edges of the box the inter quartile range (IQR) and the whiskers are drawn until the last containing value within 1.5*IQR, dots of individual measures plotted to show distribution N = 36 biological replicates. Significance was determined by ANOVA and post-hoc Welch two sample t-test with * P≤0.05 and ** P<0.01.