Novel functions of the luteinizing hormone/chorionic gonadotropin receptor in prostate cancer cells and patients
Fig 1
Expression of LHCGR in normal prostate, PCa and PCa cell lines.
(A) RT-PCR expression of LHCGR in prostate cancer cell lines, normal testis and normal prostate. Note, a distinction is made between a primer pair targeting exon 1 to 4 and a primer pair targeting exon 11 of the LHCGR. Marked with the red line are the expected and sequenced bands. 5μl cDNA loaded for both LHCGR primer pairs and 2μl cDNA loaded for RPS20 (B) Western blot with antibodies targeting the extracellular domain and the intra cellular domain of LHCGR. (C) IHC expression of LHCGR in PCa tissue samples and cell lines (LNCaP, PC-3 and DU145). Testis tissue sample is included as a positive control.