SLAMF1 contributes to cell survival through the AKT signaling pathway in Farage cells
Fig 4
Increase of Bim might underlie slightly increased apoptosis in SLAMF1 mutant cells at day 5.
(A) Cell images of SLAMF1 wild-type and mutant (8C) cells at day 5. Wild-type cells were kept aggregated together, whereas mutant cells became separated. (B) Analysis of apoptotic cells using FACS after staining cells with anti-Annexin V and propidium iodide (PI). Double positive region (upper right) indicates late apoptotic cells (Annexin V-FITC+ and PI+). (C) SLAMF1 mutant and wild-type Farage cells were seeded at 2×105 cells/mL and lysed 2 and 5 days after culture. Western blotting for phospho-AKT (S473), phospho-NF-kB p65, BCL2, MCL1, BCL-xL, and Bim at days 2 and 5.