MITF and TFEB cross-regulation in melanoma cells
Fig 3
MITF and TFEB modulate each other’s expression upon knockdown in 501Mel and Skmel28 cells.
(A) The expression of MITF and TFEB as determined by RT-qPCR after siRNA-mediated knockdown of each factor compared to control siRNA in 501Mel cells. Bars represent SEM. * indicates significance at p<0.05. (B) Western blot analysis of MITF and TFEB proteins after siRNA-mediated knockdown of MITF or TFEB in 501 Mel cells. Shown is a representative figure and quantification of three independent experiments (C). Bars represent SEM. * indicates significance at p<0.05. (D) The expression of MITF and TFEB as determined by RT-qPCR after doxycycline-induced (1μg/ml) miR-MITF knockdown, compared to miR-NTC control in Skmel28 cells; three independent experiments are shown. Bars represent SEM. * indicates significance at p<0.05.