Evaluation of transplantation sites for human intestinal organoids
Fig 2
Grafts harvested from both sites had similar physical characteristics.
(A) Gross images of representative k-tHIO (left) and m-tHIO (right). (B) Bar graph of the approximate graft width for k-tHIO and m-tHIO (n = 17 and n = 22, respectively; p = n.s.). (C) Representative images of hematoxylin and eosin stained sections of HIO Grades 1–4. Epithelial architecture is enhanced from Grades 1–4. Scale bars = 50 μm. (D) Bar graph of the number of lumens in a 2D cross-section of k-tHIO and m-tHIO (n = 17 and n = 22, respectively; p = 0.0036). (E) Histogram of lumen grades observed within k-tHIO (left) and m-tHIO (right). (n = 61 and n = 41, respectively). (F) Representative Grade 4 image of hematoxylin and eosin stained section of k-tHIO. Scale bar = 50 μm. (G) Representative Grade 4 image of hematoxylin and eosin stained section of m-tHIO. Scale bar = 50 μm. (H) Bar graph of average villus height in k-tHIO and m-tHIO (n = 6 and 9, respectively; p = n.s.). (G) Bar graph of average crypt depth in k-tHIO and m-tHIO (n = 6 and n = 9, respectively; p = n.s.). (H) Bar graph of crypt fission in k-tHIO and m-tHIO (n = 6 and n = 9, respectively; p = n.s.). H-J considered lumens of Grades 3 and 4.