Protein-conformational diseases in childhood: Naturally-occurring hIAPP amyloid-oligomers and early β-cell damage in obesity and diabetes
Fig 4
A. Neurotoxicity of RIAO from representative PTS of sera of patients and controls. Dose-dependent (7.5 = 0.029 mg/ml and 15 = 0.059 mg/ml) toxicity effects of RIAO on cerebellar granule cells (NGCs) as measured by the calcein-AM method. K25 is considered the optimal condition for NGC survival, while K5 induces more than 50% cellular death after 24 h. Codes of samples: CMN81:obesity; INP59:T1DM; CMN136:healthy child. B. Neurotoxicity of RIAO from representative PTS of patients and controls. Dose-dependent (7.5 = 0.029 mg/ml and 15 = 0.059 mg/ml) toxicity effects of RIAO on cerebellar granule cells (NGC) as measured by MTT. C. Neurotoxicity of RIAO from representative PTS of patients and controls. Dose-dependent (7.5 = 0.029 mg/ml and 15 = 0.059 mg/ml) toxicity effects of RIAO on cerebellar granule cells (NGC) as measured by calcein-AM methods. K25 is considered the optimal condition for NGC survival, while K5 induces more than 50% of cellular death after 24 h. Codes of samples: CMN136:healthy child; CMN81:obesity; CMN67:; INP23:T1DM; INP59: T1DM; INP39:T1DM.