The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster
Fig 4
Inhibition of larval growth and survival by lov knockdown in the tracheae is epistatic to overexpression of Myc.
A. Larval lengths at death or pre-pupation. For the measurement protocol see Material and methods. Control btl Gal4 /+ larvae and btl-Gal4 larvae overexpressing Myc or Myc with mCherry (control genotype for comparison to Myc; lov RNAi) survive to pupate and were measured at six days AEL. btl-Gal4 > lov RNAi, btl Gal4 > lov RNAi/mCherry and btl-Gal4 > Myc; lov RNAi larvae were measured at death (two-three days AEL). Larvae expressing both lov RNAi and Myc grew and survived only marginally better than larvae expressing lov RNAi alone. ***—p values from Student’s t test for the two critical comparisons are p = 2 x10-12 (lov RNAi/mCherry versus Myc; lov RNAi) and 2 x10-58 (Myc; mCherry versus Myc; lov RNAi). Error bars = SEMs. B. Larval numbers for the analyses. C. Representative larvae for the three critical genotypes in A. Direct white light tungsten illumination was used for imaging. This produced variable coloration at different lamp intensities. Malpighian tubules and gut are visible in the larvae in A. and C. Scale bars = 1mm.