The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster
Fig 3
lov acts downstream of Myc in larval behavioral assays.
cut(ue)-Gal4 > lov RNAi larvae are hypoxic and fail to burrow into their food and to tunnel in a substratum during the wandering phase. In contrast, cut(ue)-Gal4 > Myc larvae show normal burrowing and tunneling. Larvae (10 per plate) were assayed to determine whether the lov RNAi or Myc phenotype dominates. Petri plates with a soft agar layer and a central hole filled with yeast paste food were used as in our published protocol [26]. A. Burrowing activity. cut(ue)-Gal4 > lov RNAi larvae (~60% total) are outside their food during larval feeding. Numbers decline as larvae transition to pupae. The behavior of the test cut(ue)-Gal4 >Myc; lov RNAi larvae is very similar to that of cut(ue)-Gal4 > lov RNAi larvae. The behaviors of two control genotypes (cut(ue)-Gal4 >mRFP; lov RNAi and cut(ue)-Gal4 > Myc; GFP (each 15 copies UAS total) establish that Gal4 levels are not limiting in the test larvae. B. Tunneling behavior. Assay plates were imaged to reveal tunnels produced during wandering phase. cut(ue)-Gal4 > lov RNAi larvae show a complete absence of tunneling and larvae with the added presence of 5xUAS-mRFP have very similar activity. The test cut(ue)-Gal4 > 5xUAS-Myc; lov RNAi larvae show the minimal tunneling seen for the cut(ue)-Gal4 > 5xUAS-mRFP; lov RNAi control larvae. C. Quantitation of tunneling behavior. NIH Image J was used to quantitate tunneling in the assay plates shown in B.