Tumor-infiltrating CD62L+PD-1-CD8 T cells retain proliferative potential via Bcl6 expression and replenish effector T cells within the tumor
Fig 2
Antigen-primed CD62LintCD44high T cells in tumor-draining lymph nodes give rise to CD62L+ and CD62L- tumor-infiltrating T cells.
(A) Experimental design. CSFE-labeled OT-1 naïve T cells (CD45.2/CD45.1) were adoptively transferred into C57BL/6 (CD45.2) mice that had been transplanted with LLC-OVA 7 days previously. (B) Representative flow cytometry plots of three independent experiments. CD8+CD45.1+ tumor-draining lymph node cells were divided into five populations (I–V) based on the expression levels of CD62L and CD44. (C) Cell division of transferred OT-1 T cells in the fractions shown in (B) was measured by CFSE dilution. (D) CD69 expression in cells in the fractions was analyzed. (E) Experimental design. Draining lymph node cells in CD62LintCD44high (fr.III) from LLC-OVA transplanted OT-1 mice (CD45.2) on day 7 were sorted. Cells were adoptively transferred into wild-type mice (CD45.1) that had been transplanted with LLC-OVA 7 days previously. Tumor-infiltrating cells were analyzed on day 7. (F) Representative flow cytometry plots of tumor-infiltrating cells of three independent experiments are shown.