Different types of cartilage neotissue fabricated from collagen hydrogels and mesenchymal stromal cells via SOX9, TGFB1 or BMP2 gene transfer
Fig 2
Histological appearance of MSC hydrogels after chondrogenic differentiation by adenoviral gene transfer of GFP, SOX9, TGFB1 or BMP2.
MSCs in monolayer cultures were transduced with Ad.GFP (Control; a), Ad.SOX9 (b), Ad.TGFB1 (c) or Ad.BMP2 (d) at 5 x 102 vp/cell for each vector as indicated, seeded into hydrogel aggregates 24 h post infection and cultured in serum-free medium for 21 days. Representative examples after 21 days are shown, that were stained with H & E for assessment of cell morphology and cellularity. (a-d) Panels are shown at low (50x; bar = 200 μm) or high (200x; bar = 50 μm) magnification as indicated.