Whole-blood transcriptome profiling reveals signatures of metformin and its therapeutic response
Fig 1
Metformin-induced alterations of gene expression profiles.
(A)—Heat map and hierarchical clustering of 28 DEGs identified. Each row corresponds to one subject in the respective time-point and each column represents a DEG. Normalized sequence read counts were rescaled to lie in the range [0,1] and further used to estimate the difference between the gene expression levels in two time-points. DEGs with analogous expression values were clustered at the column level. (B)—Volcano plot showing the distribution of gene expression in the analyzed contrast. Significance versus log2 fold change is plotted on the y and x axes, respectively, calculated using likelihood ratio test and edgeR. Red dots represent the significant DEGs (FDR < 0.05), black dots–non-significant genes. M0 –time point of blood collection before administration of metformin; M3m –time point of blood collection after metformin therapy for three months.